2024
Self-organization of mortal filaments and its role in bacterial division ring formation. C Vanhille-Campos, KD Whitley, P Radler, M Loose, S Holden, A Šarić. Nature Physics.
Molecular motor tug-of-war regulates elongasome cell wall synthesis dynamics in Bacillus subtilis. S Middlemiss, M Blandenet, DM Roberts, A McMahon, J Grimshaw, JM Edwards, Z Sun, KD Whitley, T Blu, H Strahl, S Holden. Nature Communications 15, 5411
Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis. KD Whitley, J Grimshaw, DM Roberts, E Karinou, PJ Stansfeld, S Holden. Nature Microbiology 9, 1064-1074
2022
High-resolution imaging of bacterial spatial organisation with Vertical Cell Imaging by Nanostructured Immobilisation (VerCINI). KD Whitley, S Middlemiss, C Jukes, C Dekker, S Holden. Nature Protocols 17, 847-869
2021
Kinetic and structural mechanism for DNA unwinding by a non-hexameric helicase. SP Carney*, W Ma*, KD Whitley, H Jia, TM Lohman, Z Luthey-Schulten, YR Chemla. Nature Communications 12, 7015
FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation in Bacillus subtilis cell division. KD Whitley*, C Jukes*, N Tregidgo, E Karinou, P Almada, Y Cesbron, R Henriques, C Dekker & S Holden. Nature Communications 12, 2448
2019
Regulation of Rep helicase unwinding by an auto-inhibitory subdomain. MA Makurath, KD Whitley, B Nguyen, TM Lohman, YR Chemla. Nucleic Acids Research 47 (5), 2523-2532
2018
Free-energy simulations reveal molecular mechanism for functional switch of a DNA helicase. W Ma, KD Whitley, YR Chemla, Z Luthey-Schulten, K Schulten. eLife 7, e34186
Ultrashort nucleic acid duplexes exhibit long wormlike chain behavior with force-dependent edge effects. KD Whitley, MJ Comstock, YR Chemla. Physical Review Letters 120 (6), 068102
2017
Single-molecule studies of mechanical and helicase-catalyzed disruption of nucleic acid duplexes. KD Whitley. University of Illinois at Urbana-Champaign (PhD Thesis)
Elasticity of the transition state for oligonucleotide hybridization. KD Whitley, MJ Comstock, YR Chemla. Nucleic Acids Research 45 (2), 547-555
High-resolution “Fleezers”: Dual-trap optical tweezers combined with single-molecule fluorescence detection. KD Whitley, MJ Comstock, YR Chemla. Optical Tweezers, 183-256
High-resolution optical tweezers combined with single-molecule confocal microscopy. KD Whitley, MJ Comstock, YR Chemla. Methods in Enzymology 582, 137-169
2015
Direct observation of structure-function relationship in a nucleic acid–processing enzyme. MJ Comstock, KD Whitley, H Jia, J Sokoloski, TM Lohman, T Ha. Science 348 (6232), 352-354
2012
Tyrosine triad at the interface between the Rieske iron–sulfur protein, cytochrome c1 and cytochrome c2 in the bc1 complex of Rhodobacter capsulatus. JA Kyndt, JC Fitch, RE Berry, MC Stewart, K Whitley, TE Meyer, FA Walker, MA Cusanovich. Biochimica et Biophysica Acta – Bioenergetics 1817 (5), 811-818